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Similarly, bile acid treatment rescued microbial diversity and restored 5-HT, DA, acetylcholine, BDNF, and tropomyosin receptor kinase B levels in both brain and gut of zebrafish, alleviating behavioral symptoms (Qian et al., 2025)

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Such models are used to evaluate receptor activation patterns, biomarker expression, and signaling feedback mechanisms under controlled laboratory conditions, with emphasis on reproducibility and mechanistic interpretation rather than applied outcomes